Streptavidin - Pharmavidin.com

Streptavidin - Pharmavidin.com

Definition of STREPTAVIDIN

A protein similar to avidin that is produced by a bacterium of the genus Streptomyces (S. avidinii), has four identical subunits that each bind tightly to a molecule of biotin, and is used especially in the detection of molecules (as nucleic acids or antibodies) linked to a biotin label

Monomeric Streptavidin (ribbon diagram) with bound biotin (spheres)

Type OF STREPTAVIDIN

1.      Dynabeads® M-280 Streptavidin

2.      Dynabeads® My One™ Streptavidin T1

Description

Streptavidin-coupled Dynabeads® is the gold standard for isolation and handling of biotinylated molecules and targets (e.g. antibodies, proteins, peptides, small molecules, sugars, lectins, oligonucleotides and DNA/RNA).

The beads are available with different sizes and surface properties, This low-cost Trial Kit allows you to test the flexible Dynabeads® in your application

Applications:

The very high binding affinity of the Streptavidin-biotin interaction (Kd=10-15) is utilized in a vast number of applications. Over the past 15 years, Streptavidin-coupled Dynabeads® have been widely used and cited. Examples include direct/indirect capture, separation and downstream handling of nucleic acids, purification of DNA/RNA binding proteins, proteins/peptides, as well as protein interaction studies, immunoprecipitation, immunoassays, phage display, biopanning, drug screening and cell isolation. Did you know that Dynabeads® are employed on more than 25,000 routine IVD

Due to its RYD segment, STREPTAVIDIN generates number of false positive results where Neutralite avidin doesn’t. This absence of RYD segment in Neutralite avidin improves drastically the quality of the IVD’s.

 Streptavidin is a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7). With a dissociation constant (Kd) on the order of ≈10−14 mol/L,[1] the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature

Properties and Usage

Storage Temperature 4°C     Storage Conditions 0.1% BSA
0.05% Tween® 20 0.05% NaN3    1X PBS       pH 7.4 @ 25°C

Can be regenerated no Shipping Notes 4°C

Notes

Beads will bind greater than 1000 pmoles of free biotin per mg. Beads will bind greater than 500 pmoles of single-stranded 20 bp biotin oligonucleotides per mg.

Support Matrix: 1 µM nonporous super paramagnetic micro particle.

References

He, M. and Taussig, M. (1997). Nucl. Acids Res.. 25, 5132-5134.

Cuddy, K. et al. (1993). Nucl. Acids Res.. 21, 2281.

More Detail Visit :  http://www.pharmavidin.com/index.php/streptavidin

The benefit of the absence of the RYD segment with Neutralite avidin is an increase of the IVD’s quality by a drastic reduction of false positive results.

The latter is responsible for an equivocal interaction between the bacterial Streptavidin and some macromolecular components in many experimental systems. The mechanism of this interaction remained a puzzle for many years, until Alon et al. (1990) showed that Streptavidin contains an Arg-Tyr-Asp (RYD) sequence highly reminiscent of the universal cell surface recognition sequence (Arg-Gly-Asp) present in a variety of adhesion molecules, e.g. fibronectin, vitronectin, fibrinogen, and collagen. Consequently, whereas Streptavidin interacts strongly and in a biotin-independent manner with the integrins and related cell surface receptors.

Author Bio :

Pharmavidin.com Due to its RYD segment, Streptavidin generates number of false positive results where Neutralite avidin doesn’t. This absence of RYD segment in Neutralite avidin improves drastically the quality of the IVD’s. more details’ http://www.pharmavidin.com/index.php/streptavidin