Definition of STREPTAVIDIN
A
protein similar to avidin that is produced by a bacterium of the genus Streptomyces (S. avidinii), has four
identical subunits that each bind tightly to a molecule of biotin, and is used
especially in the detection of molecules (as nucleic acids or antibodies)
linked to a biotin label
Monomeric Streptavidin (ribbon diagram) with bound biotin
(spheres)
Type OF STREPTAVIDIN
1. Dynabeads® M-280
Streptavidin
2. Dynabeads® My One™
Streptavidin T1
Description
Streptavidin-coupled
Dynabeads® is the gold standard for isolation and handling of biotinylated
molecules and targets (e.g. antibodies, proteins, peptides, small molecules,
sugars, lectins, oligonucleotides and DNA/RNA).
The beads are available with different sizes and surface properties, This low-cost Trial Kit allows you to test the flexible Dynabeads® in your application
Applications:
The beads are available with different sizes and surface properties, This low-cost Trial Kit allows you to test the flexible Dynabeads® in your application
Applications:
The very high binding affinity of the Streptavidin-biotin interaction (Kd=10-15) is utilized in a vast number of applications. Over the past 15 years, Streptavidin-coupled Dynabeads® have been widely used and cited. Examples include direct/indirect capture, separation and downstream handling of nucleic acids, purification of DNA/RNA binding proteins, proteins/peptides, as well as protein interaction studies, immunoprecipitation, immunoassays, phage display, biopanning, drug screening and cell isolation. Did you know that Dynabeads® are employed on more than 25,000 routine IVD
Due to its RYD segment, STREPTAVIDIN generates
number of false positive results where Neutralite avidin doesn’t. This absence
of RYD segment in Neutralite avidin improves drastically the quality of the
IVD’s.
Streptavidin is a 60 kDa
protein purified from the bacterium Streptomyces avidinii. Streptavidin
homo-tetramers have an extraordinarily high affinity for biotin (also known as
vitamin B7). With a dissociation constant (Kd) on the order of ≈10−14 mol/L,[1]
the binding of biotin to streptavidin is one of the
strongest non-covalent interactions known in nature
Properties and Usage
Storage Temperature
4°C Storage Conditions 0.1% BSA
0.05% Tween® 20 0.05% NaN3 1X PBS pH 7.4 @ 25°C
0.05% Tween® 20 0.05% NaN3 1X PBS pH 7.4 @ 25°C
Can be regenerated
no Shipping Notes 4°C
Notes
Beads will bind
greater than 1000 pmoles of free biotin per mg. Beads will bind greater than
500 pmoles of single-stranded 20 bp biotin oligonucleotides per mg.
Support Matrix: 1
µM nonporous super paramagnetic micro particle.
References
He, M. and
Taussig, M. (1997). Nucl. Acids Res.. 25, 5132-5134.
Cuddy, K. et al.
(1993). Nucl. Acids Res.. 21, 2281.
More Detail Visit
: http://www.pharmavidin.com/index.php/streptavidin
The
benefit of the absence of the RYD segment with Neutralite avidin is an increase
of the IVD’s quality by a drastic reduction of false positive results.
The latter is
responsible for an equivocal interaction between the bacterial Streptavidin and
some macromolecular components in many experimental systems. The mechanism of
this interaction remained a puzzle for many years, until Alon et al. (1990)
showed that Streptavidin contains an Arg-Tyr-Asp (RYD) sequence highly
reminiscent of the universal cell surface recognition sequence (Arg-Gly-Asp)
present in a variety of adhesion molecules, e.g. fibronectin, vitronectin,
fibrinogen, and collagen. Consequently, whereas Streptavidin interacts strongly
and in a biotin-independent manner with the integrins and related cell surface
receptors.
Author Bio :
Pharmavidin.com Due to its RYD
segment, Streptavidin generates number
of false positive results where Neutralite avidin doesn’t. This
absence of RYD segment in Neutralite avidin improves drastically the quality of
the IVD’s. more details’ http://www.pharmavidin.com/index.php/streptavidin
